Office of Biological Safety

Sample Protocols for Containing Biohazardous Spills

The following sample protocols are provided to facilitate emergency planning and should be modified to cover the potentially biohazardous materials used in each laboratory. These instructions should be displayed in the laboratory and periodically reviewed with personnel. Download PDF copy

BIOHAZARDOUS SPILLS - OUTSIDE Containment (Biological Safety Cabinet)

• Alert co-workers and leave lab area immediately.

• Close door, post lab with "Do Not Enter" sign.

• Remove and put contaminated garments into a container for autoclaving.

• Wash hands/face with soap/antimicrobial agent.

• Notify PI/supervisor.

• Wait at least 30 minutes before re-entry (to allow aerosols to dissipate).

• Upon re-entry, wear PPE (disposable gown, mask, gloves, etc.).

• Carefully, pour an appropriate disinfectant solution (1:10 dilution of household bleach) around spill and cover spill with disinfectant soaked paper towels.  Let stand for at least 20 minutes (taking care to not create aerosols while pouring).

• Transfer all contaminated materials into an autoclave bag.

• Wash and mop entire area with appropriate disinfectant.

• Remove & discard PPE. Autoclave.

• Shower or wash hands with soap/antimicrobial agent.

• Report incident to the BSO (Biological Safety Officer), Occupational Health and the PI (if not already contacted).

BIOHAZARDOUS SPILLS - INSIDE Containment (Biological Safety Cabinet)

• Immediately stop all work, but leave BSC blower fan on during clean-up.

• Wear PPE (gloves, lab coat, eye protection).

• Pour an appropriate disinfectant solution around spill and cover spill with disinfectant soaked paper towels.

• Using paper towels and appropriate disinfectant detergent, wipe down walls, work surfaces and equipment.

• Flood work surface & drain pan (type II, BSC) with disinfectant allow to stand at least 20 minutes.

• Wipe up all excess disinfectant.

• Autoclave all contaminated materials.

• Report incident to the PI (if not already contacted).